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KMID : 0043320140370030404
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Archives of Pharmacal Research
2014 Volume.37 No. 3 p.404 ~ p.411
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Silica-based cerium (III) chloride nanoparticles prevent the fructose-induced glycation of α-crystallin and H2O2-induced oxidative stress in human lens epithelial cells
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Yang Jin
Cai Lei
Zhang Sen
Zhu Xiangjia
Zhou Peng
Lu Yi
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Abstract
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This study aimed to investigate whether silica-cerium (III) chloride (CeCl3) nanoparticles could inhibit the formation of advanced glycation end-products (AGEs) and reduce oxidative stress. Silica-CeCl3 nanoparticles were synthesised by adsorption and embedment with micro-silica materials, forming uniform nanoparticles with a diameter of approximately 130 nm. Chaperone activity assays and AGEs formation assays, and intracellular reactive assays were adopted in this study to evaluate CeCl3 nanoparticles effect. UV?visible spectrometry showed that silica-CeCl3 nanoparticles at low concentrations rapidly formed tentatively stable conjugations with α-crystallin, greatly enhancing the chaperone activity of α-crystallin. Moreover, silica-CeCl3 nanoparticles markedly inhibited the fructose-induced glycation of α-crystallin, showing an advantage over the control drugs aminoguanidine and carnosine. Silica-CeCl3 nanoparticles also reduced intracellular reactive oxygen species production and restored glutathione levels in H2O2-treated human lens epithelial cells. These findings suggest that silica-CeCl3 may be used as a novel agent for the prevention of cataractogenesis.
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KEYWORD
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α-Crystallin, AGEs, Silica-CeCl3 nanoparticle, Chaperone activity, Cataractogenesis, Glycation
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